Sweedler J. Single Cell 'Omics of Neuronal Cells 2022

Textbook in PDF format

This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
Single Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping
Single-Cell Genomic and Epigenomic DNA Analyses
Single-Cell Proteome Profiling of Neuronal Cells
Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with totalVI
Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology
High-Throughput Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells
Single-Cell Resolution Immunoblotting
Quantifying Enzyme Activity and Gene Expression Within Single Cells Using a Multiplexed Capillary Electrophoresis Platform
Quantitative Chemical Imaging at the Cellular Level:​ SIMS, Fluorescence, and Correlative Techniques
Electrophysiolog​y and Single Cells
Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells