Gryczynski Z. Practical Fluorescence Spectroscopy 2020

Textbook in PDF format

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.
Theory of Light and Light Interaction with Matter
Experimental Basics
Steady State Experiments—Transmission/Absorption
Fluorescence—Steady-State Phenomena
Steady-State Fluorescence: Applications
Steady-State Fluorescence Polarization: Anisotropy
Fluorescence: Time-Resolved Phenomena
Advanced Experiments